Figure 5.

Organotypic progenitor cell differentiation into myofibroblasts. Autologous heart, kidney, and lung anchorage-independent cells were isolated from mice, differentiated toward the myofibroblastic lineage, and prepared for immunofluorescence (n ≥ 4). Representative micrographs of heart (top), kidney (middle), and lung (bottom) are shown. Differentiated myofibroblasts at day 80 in culture were stained with DAPI (blue), the actin binding drug phalloidin (ACTB; green), and labeled for FN1 (red) and COL1A1 (gray) proteins. Scale bars = 20 μm. Abbreviations: ACTB, β-actin; COL1A1, collagen type 1 α 1; DAPI, 4′6-diamidino-2-phenylindole; FN1, fibronectin-1.