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. 2014 Sep 17;3(11):1262–1268. doi: 10.5966/sctm.2014-0086

Figure 2.

Figure 2.

The MARCM approach allows tracking of glioma of clonal origin. We established a system to positively mark clones induced specifically in the glial cells in the Drosophila brain using the MARCM approach. This system drives the expression of transgenes (e.g., UAS GFP, UAS RasV12) specifically in the glial cells and also causes recombination specifically in the glial cells because the expression of the flippase enzyme is under the control of the repo promoter (repo-flp). (A): Dorsal lobe of wild-type mature third instar larva stained for antibodies to Repo can be compared with (C): dorsal lobe of repo-Gal4 UAS GFP, which shows the expression of the repo-Gal4 transgene using UAS GFP reporter expression. Images in (A, C) show that the repo-Gal4 driver is capable of driving transgenes in all glial cells (red in [A]) in the brain. (B, D): Examples of positively marked clones are shown. Higher magnification images in (B′) and (D′) show the effects of clones early (day 5) and later (day 7) in development. Magnification, ×40 (A, C, B′, D′), ×20 (B, D). Abbreviation: MARCM, mosaic analysis with a repressible cell marker.