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A two-stage microfluidic device with improved antibody density on the device surface enriched CD34+ stem cells in high purity. When antibody was rearranged and concentrated on the top surface of hydrogel inside the second-stage microfluidic device, the purity of collected CD34+ stem cells reached 82 ± 2% (mean ± SD). This purity level is significantly higher than our previous two-stage device design [22] (n = 4 per group, p < .01). Abbreviation: CD34+, CD34-positive.
