Groups C, D, and E had visible hair outgrowth from the skin defect sites. Group D, with the injection of both microfluidic-enriched CD34-positive stem cells and unenriched epidermal cells, had the most extensive hair growth among all groups. Without the epidermal cell population, the wound healed in groups A and B, but no hair was formed. Group F shows the extreme case of natural hair in Nu/Nu without surgery. A thin layer of hair can be seen on the dorsal skin of nude mice.