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. 2014 Sep 17;3(11):1331–1341. doi: 10.5966/sctm.2014-0120

Figure 2.

Figure 2.

Differential localization of niche cells in explant culture (EC) and suspension culture (SC) systems. (A): Phase image of growing cultures established by EC and SC methods. (B): Localization of nestin-positive cells (red) at the EE in the EC system. C/EBPδ expression is shown in green. (C): Localization of nestin-positive cells (green) at the LE in the SC system. Expression of stromal cell markers in EC and SC systems. (D): Expression of C/EBPδ (green) in a rare subset of nestin-positive cells (red) at the EE in an EC system. (E): Coexpression of p27 (green) in all nestin-positive cells (red). (f) Expression of vimentin (green) both by the epithelial cells and the stromal cells migrating at the LE in an SC system. (G): Exclusive expression of CD29 (green) by the limbal epithelial cells, whereas the nestin-positive cells (red) are seen migrating at the colony periphery. The cells were counterstained with propidium iodide (PI) to label the nuclei in red. Arrows indicate the EE and LE boundaries. Arrowheads mark the cells expressing different antigens. Abbreviations: EC, explant culture; EE, explant edge; LE, leading edge; SC, suspension culture.