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. 2014 Sep 17;3(11):1331–1341. doi: 10.5966/sctm.2014-0120

Figure 4.

Figure 4.

Localization of slow-cycling stem cells in limbal explant cultures. (A): Epithelial cells that retained BrdU (green) after a 1-hour pulse and a 10-day chase. Note the localization of BrdU-positive cells at both the EE and the LE of explant cultures. (B): Note the intense and uniformly labeled slow-cycling cells that are prominent at the LE. (C): BrdU label (red) retaining cells at the LE coexpressing p15 (green). (D): BrdU label (red) retaining cells at the LE coexpressing C/EBPδ (green). Magnification, ×400 (A–D). Centripetal migration of cells and self-organizing three-dimensional (3D) niche compartments in limbal cultures. (E): Suspension cultures of limbal epithelial cells migrating in whirling pattern. Note the emerging 3D-sphere clusters within the spirals. (F): 3D-sphere clusters emerging at the migrating edges or LEs and within the growing epithelial holoclones. Detailed view of 3D cell clusters at the junction of two colonies (G) and at the middle of a holoclone (H). Note the melanin pigmentation within these cell clusters. Arrows mark the clonal boundaries and junctions. Arrowheads indicate the 3D cell clusters. Magnification, ×40 (E, F), ×100 (G, H). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; EE, explant edge; LE, leading edge.