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. 2014 Sep 17;3(11):1331–1341. doi: 10.5966/sctm.2014-0120

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Figure 5. — Localization of quiescent stem cells in close proximity to the niche-like three-dimensional (3D) compartments. (A): Localization of p27 (green) and nestin (red) coexpressing niche cells within and around the 3D-sphere clusters. Note the cytosolic expression of p27 in the cells within the spheres. (B): Localization of p15 (red) and p63α (green) coexpressing limbal epithelial stem cells around the 3D-sphere clusters. ∗, Note the cell-free central core in this sphere with possible extracellular matrix components and melanin pigmentation that blocks the fluorescence signal and appear as a hollow in the center. (C): Localization of p21 (red) and p63α (green) coexpressing limbal epithelial stem cells around the 3D-sphere clusters. All images are at ×100 magnification.