Table 1.

Composition of the flux solutions

	Regular	Regular	92 Na+	10 Na+	92 Na+	10 Na+
	Isosmotic	Hyperosmotic	30 Cl−	30 Cl−	10 Cl−	10 Cl−
NaCl	96	96	22	10	2	2
NaOH	–	–	70	–	90	8
HCl	–	–	–	12	–	–
CH4O3S	–	–	70	70	90	90
C7H17NO5	–	–	–	82	–	82
KCl	4	4	4	4	4	4
			80 Na+	10 Na+	80 Na+	10 Na+
			20 K+	20 K+	2 K+	2 K+
NaCl			80	10	80	10
NaOH			–	–	–	–
HCl			–	70	18	88
CH4O3S			–	–	–	–
C7H17NO5			–	70	18	88
KCl			20	20	2	2
			45 Cl−	15 Cl−	45 Cl−	15 Cl−
			8 K+	8 K+	1 K+	1 K+
NaCl			33	3	40	10
NaOH			59	89	52	82
HCl			–	–	–	–
CH4O3S			59	89	52	82
C7H17NO5			–	–	–	–
KCl			8	8	1	1
All solutions above also contain
CaCl2	2	2	2	2	2	2
MgSO4	1	1	1	1	1	1
HEPES/C7H17NO5	10	10	10	10	10	10
Sucrose	–	65	65	65	65	65
pH	7.4	7.4	7.4	7.4	7.4	7.4
Osmolarity (mosM)	200	265	265	265	265	265

All concentrations are given in millimolars per liter. For kinetics, the solutions were mixed to provide solutions with intermediate concentrations. CH₄O₃S, methanesulfonic acid; C₇H₁₇NO₅, N-methyl-d-glucamine.