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. Author manuscript; available in PMC: 2015 Oct 30.
Published in final edited form as: Nature. 2014 Aug 24;514(7524):646–649. doi: 10.1038/nature13660

Figure 3.

Figure 3

Effects of apcin on Cdc20 binding to APC/C and stability of APC/C substrates in mitotic Xenopus extract. a, Apcin blocks co-receptor-dependent binding of Cdc20 to APC/C. Substrate (cycB1-NT, 1 μM or 10 μM), apcin and/or TAME (50 μM each) were added, and APC/C was isolated with anti-Cdc27 antibody. Levels of Cdc20 were assessed by western blotting and normalized to the levels of Cdc27. b, Effect of apcin and TAME on APC/C substrate stability. Levels of 35S-labeled substrates were assessed by gel electrophoresis and phosphorimaging. c, As in b except that combinations of apcin and TAME were examined. Values and error bars in a, b, c represent mean of three experiments +/- S.E.M.