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. Author manuscript; available in PMC: 2015 Apr 30.
Published in final edited form as: Nature. 2014 Aug 31;514(7524):633–637. doi: 10.1038/nature13637

Extended Data Figure 2. Characterization of spacer 32T isolates lysogenized with ΦNM4.

Extended Data Figure 2

a, Visualization of TB4-derived strains grown on egg-yolk agar. Integration of ΦNM4 within the geh locus of TB4 results in strongly reduced lipase secretion, enabling a screen for ΦNM4 lysogenization with spacer 32T. Right-most lanes display two lipase-negative isolates from the lysogenization screen; picture is representative of five technical replicates for each isolate. b, ΦNM2 sensitivity assay. Strains shown in panel a were re-streaked through the indicated ΦNM2-seeded region from top to bottom. The pGG3 lysogen and spacer 32T non-lysogen in the two left-most lanes serve as sensitive and insensitive controls, respectively. Picture is representative of three technical replicates for each isolate. c, ΦNM2 plaquing efficiency on soft agar lawns of the strains analyzed in panels a and b. 32T(L1) and 32T(L2) refer to the two ΦNM4 lysogens isolated during the spacer 32T egg-yolk screen. Error bars: mean ± s.d. (n=3).