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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Pharm Res. 2014 May 22;31(11):3106–3119. doi: 10.1007/s11095-014-1403-6

Fig. 3.

Fig. 3

Intracellular ROS concentrations at 1.5, 6, 24 and 48 hours in untreated PC-3 cells and PC-3 cells treated with TBHP at 100 µM, CuCl2 at 154 µM, or LpCu at 122 µM. Fluorescence intensity results are normalized with respect to total protein concentration. One representative result is shown from three independent experiments (N = 3). At 24 and 48 hours, there were statistical differences in ROS levels between untreated cells and LpCu, and untreated cells and CuCl2. There was also a significant difference in ROS levels between LpCu and CuCl2 at 24 hours, but at 48 hours the difference became insignificant. Results presented are MEAN ± SEM of arbitrary units (n = 8). * p < 0.05, **p < 0.01, ns means not significant.