Table 1.

Primers and PCR conditions used in this study.

Gene	Primer sequence	Size of product (bp)	PCR conditions	Reference
IntI1-F	GGT CAA GGA TCT GGA TTT CG	436 bp	5 min at 94°C; 32 cycles of 1 min at 94°C, 1 min at 60°C, 2 min at 72°C; 10 min at 72°C	Machado, 2005
IntI-R	ACA TGC GTG TAA ATC ATC GTC
IntI2-F	CAC GGA TAT GCG ACA AAA AGG	788 bp	5 min at 94°C; 32 cycles of 1 min at 94°C, 1 min at 60°C, 2 min at 72°C; 10 min at 72°C	Machado, 2005
IntI2-R	TGTA GCA AAC GAG TGA CGA AAT G
IntI3-F	AGT GGG TGG CGA ATG AGT G	600 bp	5 min at 94°C; 32 cycles of 1 min at 94°C, 1 min at 60°C, 2 min at 72°C; 10 min at 72°C	Machado, 2005
IntI3-R	TGT TCT TGT ATC GGC AGG TG
5'CS	GGC ATC CAA GCA GCA AG	Variable	5 min at 94°C; 35 cycles of 1 min at 94°C, 1 min at 58°C, 2 min at 72°C; 10 min at 72°C	Machado, 2005
3'CS	AAG CAG ACT TGA CCT GA
attI2-F	GAC GGC ATG CAC GAT TTG TA	Variable	5 min at 94°C; 35 cycles of 1 min at 94°C, 1 min at 58°C, 2 min at 72°C; 10 min at 72°C	Machado, 2005
orfX-R	GAT GCC ATC GCA AGT ACG AG