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. 2014 Oct 28;14:260. doi: 10.1186/s12866-014-0260-0

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© Hwang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The Haloferax volcanii THI4 homolog is required for growth in the absence of thiamine and the thiazole moiety 4-methyl-5-(β-hydroxyethyl)thiazole (THZ). Hfx. volcanii strains including H26 parent (wt), HM1052 (ΔubaA), NC1011 (Δthi4), NC1011-pJAM202c (Δthi4 + empty vector), NC1011-pJAM2821 (Δthi4 + HvThi4), and NC1011-pJAM2822 (Δthi4 + HvThi4 C165A) were grown in GMM supplemented with and without thiamine or THZ as indicated and described in the Materials and Methods. First round (A) and successive growth curves (B and C) are presented with the mean ± standard deviations shown.