Figure 3.

Endothelin receptor A blockade promotes vessel sprouting in oxygen-induced retinopathy (OIR) retinas. P12 OIR mice were injected intravitreally with vehicle (Veh) or BQ-123 (17 μg/μL per eye) and sacrificed on P17. Retinas were dissected, flat mounted, and stained with isolectin B4 (red, blood vessels), and images were taken at high magnification using confocal microscope. A: Two representative images of retinal vessel sprouting (white arrowheads) are shown for each group. B: Quantification of number of vessel sprouts reveals that OIR mice treated with BQ-123 show more vessel sprouting compared with OIR + Veh. ^∗P < 0.05. n = 6 retinas, two litters of mice (B). Scale bar = 20 μm (A).