Table 4.

PCR Primers for Confirmation of the Most Dysregulated Genes from Macro Samples

Primer name	Sequence
AGER	Forward: 5′-GATGGTGTGCCCTTGCCCCTTCC-3′
Reverse: 5′-GATCCTCCCACAGAGCCTGCAGTTG-3′
AKR1B10-UR	Forward: 5′-TGTTCAACTAGGATCAGAATATCACA-3′
AKR1C2-UR	Forward: 5′-TGTGGATGGTGACACAGAGG-3′
ANKRD22	Forward: 5′-GCTGCCTGAGCTGCTGGAAA-3′
Reverse: 5′-GCTGCTTGGCAGATGGGCTCA-3′
AQP4l	Forward: 5′-GGAATTTCTGGCCATGCTTA-3′
Reverse: 5′-AGACTTGGCGATGCTGATCT-3′
β-Actin	Forward: 5′-ACAGAGCCTCGCCTTTGCCG-3′
Reverse: 5′-CATCACGCCCTGGTGCCTGG-3′
β-Actin UR	Forward: 5′-TATTTTGAATGATGAGCCTTCGT-3′
CEACAM5	Forward: 5′-GGTGCATCCCCTGGCAGA-3′
Reverse: 5′-GGTTGCCATCCACTCTTTCA-3′
CYP24A	Forward: 5′-ACCCAGGTGTTGGGATCCAGTGA-3′
Reverse: 5′-AGCTCTGCTAATCGGCGACCA-3′
CYP24A1	Forward: 5′-ACCCAAAGGAACAGTGCTCATGCT-3′
Reverse: 5′-TGCGGACAATCCAACAAAGAGCCA-3′
DSC3-UR	Forward: 5′-GCCAGAATGCTTGGATATGAA-3′
DSG3	Forward: 5′-CGACCGGGAGGAAACTCCAAGC-3′
Forward-2: 5′-AGCTGCTAGCTTGCTCTCGGTCA-3′
Reverse: 5′-GTGCCTCAAACTCACTGGTGATGA-3′
Reverse-2: 5′-TCATCACCAGTGAGTTTGAGGCAC-3′
FABP4	Forward: 5′-GAAGTAGGAGTGGGCTTTGCCACC-3′
Reverse: 5′-GCACATGTACCAGGACACCCCC-3′
GAPDH-U	Forward: 5′-AGCCCCAGCAAGAGCACAA-3′
HHIP	Forward: 5′-GCGGATGAGTTTTGCTTTTA-3′
Reverse: 5′-AGCCATCCCCACTATGC-3′
KRT6a	Forward: 5′-CCTTCATCGACAAGGTGCGGT-3′
Reverse: 5′-CACCAGGTCCTGCATGCCTCTG-3′
MMP1	Forward: 5′-GGGAGATCATCGGGACAACTC-3′
Reverse: 5′-GGGCCTGGTTGAAAAGCAT-3′
MMP12	Forward: 5′-TGCTGATGACATACGTGGCA-3′
Reverse: 5′-AGGATTTGGCAAGCGTTGG-3′
Muc21	Forward: 5′-CTTCCCATAGTGCATCTACTGC-3′
Reverse: 5′-AGGGACAGGCTGTTTCTCAC-3′
NAP5	Forward: 5′-GGGAAGAACCTCAGGAAAAGGCAAT-3′
Reverse: 5′-TGGGGGTTCAAGTAACACTTTTCGC-3′
RTKN2	Forward: 5′-GCCGACTAGTTGCCCAGCCA-3′
Reverse: 5′-TGCCCGGATTCTGGTTTCCTTGT-3′
RTKN2-UR	Forward: 5′-GGAGAAAATACTAATGCTGACACG-3′
SERPINB5-UR	Forward: 5′-GTGTTGCAGGTTCATGGATT-3′
SPINK1	Forward: 5′-ACTCCCTGGGAAGAGAGGCCAAAT-3′
Reverse: 5′-GGCCTCGCGGTGACCTGATG-3′
SPP1	Forward: 5′-ACAGCCAGGACTCCATTGA-3′
Reverse: 5′-TCAGGTCTGCGAAACTTCTTAG-3′
TMPRSS4	Forward: 5′-CCGATGTGTTCAACTGGAAG-3′
Reverse: 5′-GAGAAAGTGAGTGGGAACTG-3′
UR60	Reverse: 5′-AACGAGACGACGACGGACTTT-3′
WI	Forward: 5′-CAGTGCCTCACAAGGCATCAGTTGT-3′
WIF1	Reverse: 5′-CTCCATTTCGGCACCCGCCT-3′

Universal reverse PCR primer (UR), amplification with the Spivack Laboratory’s RNA-specific RT-qPCR strategy with tagged primer to avoid inadvertent genomic DNA pseudogene amplification.¹³