Fig. 3.

Protein identification by MS analysis. RBCs were treated with or without 500 μM t-BHP and proteins contained in fractions corresponding to peaks a and c in Fig. 1 were prepared. The proteins were separated using 12% SDS–PAGE and detected by silver staining. Protein bands No. 1 and No. 2 were excised and subjected to in-gel digestion and PMF using MALDI–TOF–TOF-MS. Proteins derived from bands No. 1 and No. 2 were identified as human Prx2 (Accession; P32119).