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. Author manuscript; available in PMC: 2015 Apr 28.
Published in final edited form as: Nat Commun. 2014 Oct 28;5:5270. doi: 10.1038/ncomms6270

Figure 1. Genetic analysis of PhoP-regulated circuits.

Figure 1

(a) PhoP is activated via phosphorylation by the membrane-associated sensor-kinase, PhoQ, in response to low extracellular Mg2+ or cationic antimicrobial peptides at acidic pH. Phosphorylated PhoP (PhoP P) up-regulates phoP and pagC. Up-regulation of pagC also requires SlyA and repression by H NS. RT-PCR analysis of pagC (b) and phoP (c) mRNA under inducing (10 μM Mg2+) and non-inducing (10 mM Mg2+) conditions. Data represent the mean ± SEM; n = 3. Other PhoP regulon members gave similar results (Supplementary Fig. S1).