Figure 2. PhoP-mediated activation is dependent on promoter architecture.

In vitro transcription was performed on each of the PhoP target genes described in Fig. 1 and Supplementary Fig. 1 in the presence of increasing concentrations of PhoP (black line) and PhoP-P (green line). Only class A genes are activated by PhoP-P. Transcriptional output is normalized to the 0 nM reaction. Data represent the mean ± SEM; n = 3 for all genes except ompX, pagK, and rstA, where n=4.