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. 2014 Nov 1;28(21):2381–2393. doi: 10.1101/gad.250985.114

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© 2014 Schönemann et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Transcriptome-wide binding of WDR33 to AAUAAA. (A) Average density of PAR-CLIP reads around the 1000 most abundantly used CP sites in HEK293 cells. WDR33 shows strong and specific positioning upstream of the CP sites, with a peak at nucleotides −16 to −18 upstream of the cleavage site. (B) Enrichment of T-to-C transition relative to the T nucleotide frequency as a function of distance with respect to the poly(A) signal. The analysis is based on the 500 most frequently used CP sites that have a single AATAAA (top panel) or ATTAAA (bottom panel) motif and no other variant poly(A) signal in the 40-nt region upstream of the cleavage site. These results indicate that WDR33 is most frequently cross-linked on U nucleotides that immediately follow the polyadenylation signal.