Figure 2.
NF1 ablation impairs GNP migration and proliferation. (A) Tracing of granule neuron migration was assessed in control and NF1hGFAP mice that were pulsed with BrdU at P7 and chased until P12. Arrowheads show an increased number of BrdU-positive cells in the mutant pial surface, indicating impaired migration. Bar, 100 μm. (B) Quantification of a decreased number of mutant BrdU-positive cells reaching the IGL. Mean ± SEM; n = 4; (***) P < 0.001. (C) Decreased EGL cell proliferation in the mutant cerebellum. GNP proliferation was evaluated after a short 2-h chase following BrdU administration at P7 in control and mutant animals. Bar, 50 μm. (D) Quantification of BrdU-positive cells among total cells in the EGL (identified with DNA staining). Mean ± SEM; n = 4; (**) P < 0.01