Figure 5.

Neuronal migration is impaired in NF1^Nes mice. (A) Assessment of neuronal migration via immunostaining with BrdU, p27 (post-mitotic cell marker), and NeuN (granule neuron marker) performed 5 d (P12) after BrdU administration (P7). In the mutant IGL, p27 and NeuN staining show a similar disorganized pattern compared with control. Bar, 50 μm. (B) Quantitative analysis showed a reduced number of BrdU and p27 double-positive cells in the NF1^Nes IGL. Mean ± SEM; n = 5; (****) P < 0.001. (C) NF1^Nes mice exhibited an increased ratio of BrdU-positive cells in the ML to BrdU-positive cells in the IGL. Mean ± SEM; n = 5; (***) P < 0.001. (D) Reduced number of mature neurons in the mutant IGL. Mean ± SEM; n = 5; (***) P < 0.001.