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. 2014 Nov 1;28(21):2421–2431. doi: 10.1101/gad.241588.114

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© 2014 Foronda et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.

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Figure 3. — miRNA sponges distinguish between miR-275 and miR-305 function. (A) esg-Gal4 was used to direct UAS-GFP alone or together with UAS sponge transgenes that deplete miR-275 or miR-305. Depletion of miR-305 increased the number of esg-Gal4-expressing ISCs and EB cells. ANOVA: P = 0.4 comparing control with miR-275 sponge and P < 0.0001 comparing control with miR-305 sponge. (B) Dl-Gal4 was used to express UAS-miR-275 or UAS-miR-305 in ISCs in the KO/KO mutant background. Dl-Gal4>UAS-GFP cells (green). DAPI (red). ANOVA: P = 0.5 comparing control with UAS-miR-275; P < 0.0001 comparing control with UAS-miR-305.