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. 2014 Nov 1;28(21):2421–2431. doi: 10.1101/gad.241588.114

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© 2014 Foronda et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.

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Figure 6. — Regulation of miR-305 by the insulin pathway. (A) qRT–PCR to measure the unprocessed miR-305 primary transcript in RNA isolated from ISCs by TU tagging. The level of miR-305 primary transcript increased versus the fed control in two independent experiments. Flies were 7 d of age at the time of TU labeling. (B,C) miR-305 sensor transgene expression. Dl-Gal4 was used to direct UAS-RFP in the ISCs (arrows). (B) Control. (C) Dl-Gal4 + UAS-RFP + UAS-PI3K or UAS-PTEN^RNAi transgenes were used to activate the IIS pathway in the ISCs and were examined at 7 d of age. (D) Ratio of Dl⁺ ISCs that showed miR-305 sensor GFP expression versus those without detectable levels of GFP for the experiment in B and C. ANOVA: P < 0.0001 for UAS-PI3K and for UAS-PTEN-RNAi compared with the control.