FIGURE 7.

Effects of ARAP2 and ACAP1 on Arf6-positive structures in cells treated with AlF₄⁻. HeLa cells transfected with Arf6-HA alone (Arf6+pCI vector) or together with FLAG-ARAP2 or FLAG-ACAP1 were treated with AlF₄⁻ for 30 min, fixed, and stained with anti-HA and anti-FLAG antibodies. A, representative images of various Arf6-positive structures by anti-HA staining. B, anti-HA staining images were scored blindly as different Arf6 structures for 20∼55 cells from each group in each experiment. The results shown are quantifications (mean ± S.E.) from two experiments. C–E, ARAP2- and ACAP1-induced structures depend on active Arf6. HeLa cells expressing [N122I]Arf6-HA with FLAG-ARAP2 or FLAG-ACAP1 were treated as in A. Anti-FLAG staining images were scored blindly for 40∼70 cells in each experiments. Representative images (C) and quantification (mean ± S.E.) from two to three experiments (D and E) are shown. Scale bars = 10 μm. *, p < 0.05; **, p < 0.01.