FIGURE 6.
Methylene blue modulates BACE1 expression and activity in CHO/APPWT cells. A, modulation of BACE1 expression in CHO/APPWT cells treated with various doses of MB. Western blots are shown using a carboxyl-terminal BACE monoclonal antibody (mAb BACE), a nicastrin polyclonal antibody (pAb nicastrin), a carboxyl-terminal PS1 monoclonal antibody (pAb PS1), and an amino-terminal presenilin enhancer 2 (PEN-2) polyclonal antibody (pAb PEN-2). β-actin is included as an internal reference control, and densitometry data are shown as ratios of β-actin below each lane. B, densitometry results are shown as ratios of BACE to β-actin at various MB doses. C, cell-free BACE1 activity assay results are displayed, and % of BACE1 activity is shown on the y axis. All statistical comparisons are between 0 μm and various doses of MB (*, p < 0.05; **, p < 0.01; ***, p < 0.001), and similar results were observed in 3–4 independent experiments.