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Organization of the PilF domains (A) and heat stability of PilF variants (B). The general secretory pathway domains are abbreviated with GSPII. The Walker A and Walker B motifs are indicated. The conserved tetracysteine motif is enlarged. The purified complexes of the PilF variants were incubated for 0, 2.5, or 5 min at 70 °C, and then the samples were cooled on ice. 35 μg of the treated samples was separated by clear-native PAGE, and the proteins were stained with Coomassie Blue (B).
