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Dissociation temperature of PilF wild-type protein in presence of different concentrations of AMP-PNP (A) or ADP (B). Calculation of the dissociation constant by subtracting the melting transition without AMP-PNP or ADP from the melting transitions with different amounts of AMP-PNP (■) or ADP (▾) (C). The values are plotted against the nucleotide concentration. Dissociation constants are calculated by Graph Pad Prism 4.
