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Effect of growth temperature on adhesion of pilF mutants. T. thermophilus cells were incubated in TM+ medium at 64 °C (A) or at 55 °C (B) in 96-well plates for 3 days. Adherence was calculated by the absorbance of crystal violet (570 nm) mediated by the adhered cells deviated by the amount of planktonic cells (600 nm). The mean was built from three independent cultures and in triplicate.
