TABLE 1.
Name and sources of the strains
Kan, kanamycin.
| Strain | Genotype | Source |
|---|---|---|
| T. thermophilus HB27 | Wild-type strain | (42) |
| ΔpilF::bleo | HB27, pilF (TTC1621) replaced by a bleomycin resistance marker, BleoR | (13) |
| wild-type | ΔpilF complemented with pDM12-pilFwt, BleoR, KanR | (13) |
| C781A (C1A) | ΔpilF complemented with pDM12-pilFC781A, BleoR, KanR | This study |
| C784A (C2A) | ΔpilF complemented with pDM12-pilFC784A, BleoR, KanR | This study |
| H783A/C784A (HC2A) | ΔpilF complemented with pDM12-pilFH783A/C784A, BleoR, KanR | This study |
| C816A (C3A) | ΔpilF complemented with pDM12-pilFC816A, BleoR, KanR | This study |
| C819A (C4A) | ΔpilF complemented with pDM12-pilFC819A, BleoR, KanR | This study |
| C781A/C784A (2CysA) | ΔpilF complemented with pDM12-pilFC781A/C784A, BleoR, KanR | This study |
| C781A/C784A/C816A (3CysA) | ΔpilF complemented with pDM12-pilFC781A/C784A/C816A, BleoR, KanR | This study |
| C781A/C784A/C816A/C819A (4CysA) | ΔpilF complemented with pDM12-pilFC781A/C784A/C816A/C819A, BleoR, KanR | This study |