TABLE 3.

Increase in melting transitions in the presence of AMP-PNP and ADP, respectively

PilF variant	ΔtAMP-PNPa	ΔtADPb
	°C	°C
Wild type	+4.0 ± 0.6	+4.9 ± 0.6
C1A	+4.6 ± 1.7	+6.2 ± 1.7
C2A	+6.6 ± 0.6	+7.5 ± 0.7
HC2A	+6.8 ± 0.6	+9.8 ± 0.2
C3A	+6.9 ± 0.8	+9.5 ± 0.7
C4A	+6.4 ± 0.3	+8.1 ± 0.3
2CysA	+7.5 ± 1.2	+8.7 ± 1.1
3CysA	+7.6 ± 0.2	+8.3 ± 0.5
4CysA	+5.8 ± 0.4	+7.6 ± 0.3

^a Δt_AMP-PNP was calculated by subtracting the melting transition without AMP-PNP from the melting transition with 5 mm AMP-PNP.

^b Δt_ADP was calculated by subtracting the melting transition without ADP from the melting transition with 2.5 mm ADP.