FIGURE 7.

β-Rich oligomer formation at pH 2.0. A, far-UV CD spectra at pH 2.0 in the absence of 150 mm NaCl (solid curve) in the presence of 150 mm without incubation (dotted line) and in the presence of 150 mm after 4 days of incubation (dashed line). B, elution profiles after variable times of incubation at pH 2.0, 150 mm NaCl. The pH 2.0 buffer (pH 2.0, 20 mm glycine, 150 mm NaCl) was utilized for the elution buffer. This experiment was performed at 20 °C with a protein concentration of 10 μm. a.u., absorbance units.