FIGURE 2.

Validation of miRNA arrays. Total RNA was isolated from fresh first trimester and term human placental tissue, and qPCR analysis was performed, using individual miRNA primer sets for hsa-miR-29a (A), hsa-let-7a (B), hsa-miR-125b (C), hsa-miR-145 (D), hsa-miR-143 (E), hsa-miR-143–3p (F), hsa-miR-377 (G), and hsa-miR-675 (H). Data were normalized to 5 S rRNA expression and are presented as median and range mRNA and were analyzed by the Mann-Whitney U test. Data were considered significant if p < 0.05. Results shown are representative of at least five independent experiments.