FIGURE 4.

miR-377, let-7a, and miR-145 are enriched in trophoblast cells. Levels of miR-377 (A), let-7a (B), and miR-145 (C) were assessed in a panel of placental trophoblast cell lines (BeWo, JAR, JEG, and SWAN-71), primary cells (primary cytotrophoblast (CT) and primary stromal cells), and fragments of whole placental tissue from term or first trimester placenta. Levels are expressed as levels of specific miR relative to 5 S rRNA. miR-377, let-7a, and miR-145 are all expressed in both primary cytotrophoblast and trophoblast cell lines, albeit at low levels.