TABLE 3.
miR-145 regulates the expression of multiple components of the IGF axis in the first trimester placenta
Pre-miR-145 (500 nm) was transfected into first trimester placental explants. 48 h later, total RNA was extracted, and mRNA expression of multiple genes was assessed using PCR pathway arrays. Expression levels were normalized to internal controls and expressed as -fold change compared with negative control pre-miR. -Fold changes ≥2 were considered significant.
| Gene | Description | Accession number | -Fold change |
|---|---|---|---|
| RPS6KA1 | Ribosomal protein S6 kinase polypeptide 1/RSK1 | NM_009097 | −11.577 |
| TSC1 | Tuberous sclerosis 1 | NM_022887 | −8.358 |
| PRKCA | Protein kinase C, α | NM_011101 | −7.029 |
| PIK3R2 | Phosphatidylinositol 3-kinase, regulatory subunit, polypeptide2 (p85 β) | NM_008841 | −6.076 |
| CCND1 | Cyclin D1 | NM_007631 | −5.749 |
| CD14 | CD14 antigen | NM_009841 | −4.801 |
| HRAS1 | Harvey rat sarcoma virus oncogene 1 | NM_008284 | −4.238 |
| TSC2 | Tuberous sclerosis 2 | NM_011647 | −3.715 |
| HSPB1 | Heat shock protein 1 | NM_013560 | −3.638 |
| RHOA | Ras homolog gene family, member A | NM_016802 | −3.466 |
| FKBP1A | FK506 binding protein 1a | NM_008019 | −3.371 |
| MAPK8 | Mitogen-activated protein kinase 8/JNK | NM_016700 | −3.325 |
| RAF1 | V-raf-leukemia viral oncogene 1 | NM_029780 | −3.017 |
| ILK | Integrin linked kinase | NM_010562 | −2.996 |
| CDKN1B | Cyclin-dependent kinase inhibitor 1B | NM_009875 | −2.894 |
| MYD88 | Myeloid differentiation primary response gene 88 | NM_010851 | −2.854 |
| MAPK14 | Mitogen-activated protein kinase 14/p38 MAPK | NM_011951 | −2.835 |
| GUSB | Glucuronidase β | NM_010368 | −2.757 |
| PDPK1 | 3-Phosphoinositide-dependent protein kinase 1/PDK1 | NM_011062 | −2.645 |
| RBL2 | Retinoblastoma-like 2 | NM_011250 | −2.591 |
| FASl | Fas ligand (TNF superfamily, member 6) | NM_010177 | −2.400 |
| ITGB1 | Integrin β1 | NM_010578 | −2.384 |
| RASA1 | RAS p21 protein activator 1 | NM_145452 | −2.005 |
| IGF1R | Insulin-like growth factor I receptor | NM_010513 | −2.001 |
| PAK1 | P21 protein (Cdc42/Rac)-activated kinase 1 | NM_011035 | 2.726 |
| GRB2 | Growth factor receptor-bound protein 2 | NM_008163 | 2.670 |
| CASP9 | Caspase-9 | NM_015733 | 2.229 |
| IRAK1 | Interleukin-1 receptor-associated kinase 1 | NM_008363 | 2.124 |
| SRF | Serum response factor | NM_020493 | 2.009 |