FIGURE 6.

ErbB2 in the juxtanuclear compartment co-localizes with PKC-α and ERC marker Arf6. A and B, SKBR-3 cells were transiently transfected to co-express GFP-tagged wild-type PKC-α, ds-Red-tagged WT Arf6 (A) or Arf6-T27N mutant (B). The cells were stained with Alexa Fluor® 647-conjugated anti-ErbB2 antibody, treated with DMSO (vehicle) or PMA (100 nm) for 4 h, paraformaldehyde-fixed and permeabilized as in Fig. 5, and imaged under a confocal microscope. C, SKBR-3 cells were transiently transfected to co-express GFP-tagged wild-type PKC-α, and ds-Red-tagged WT Arf6 or Arf6-T27N mutant as in A and B, and 40-μg aliquots of Triton X-100 lysate protein were used for Western blotting with anti-Arf6 and β-actin (loading control) antibodies. D, SKBR-3 cells transiently transfected and stained as in A were treated with PMA (100 nm) for 4 h, paraformaldehyde fixed, stained with an anti-Arf6 antibody and imaged under a confocal microscope.