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efm2Δ but not efm3Δ cells have increased stop codon read-through. Dual luciferase assays were utilized to measure the percentage of stop codon read-through, amino acid misincorporation, and frameshifting. Titles of each panel indicate the stop codon analyzed, the misincorporation of lysine, or the direction of programmed ribosomal frameshift (PRF). Values for three or four replicates with S.D. are shown as error bars. p values are displayed where differences were less than 0.05.
