FIGURE 10.

IGF-1 rescue of islet cell death is inhibited by SPARC. Dispersed islets were adhered to coverslips in serum-free medium then treated for 2 days (A) with either 10% FBS, untreated (0% FBS), 200 ng/ml IGF-1 or 200 ng/ml HGF. Islet cells were stained with FDA (green/live) and PI (red/dead) and the %live cells determined using ImageJ. The mean percentage of live islet cells ± S.E. is shown. Islets were pooled from 4 ICR mice, and n = 4 per treatment. B, islets were cultured with either 10% FBS, untreated (0% FBS), IGF-1 (200 ng/ml), SPARC (20 μg/ml), or both IGF-1 and SPARC and stained as in A. Results are pooled from three separate experiments. In both graphs the mean percentage of live islet cells ± S.E. is shown. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.