FIGURE 7.

SPARC inhibits growth factor signaling in primary mouse islets. Dispersed islets (groups of 150) were pre-incubated with SPARC (20 μg/ml for 1 h) before treatment with 20 ng/ml HGF. Cell lysates were analyzed by Western blotting using antibodies against phosphorylated and total ERK 1/2. A representative blot is shown in A. The numbers below the blot indicate band intensities standardized to total ERK 1 and relative to the control in lane 1. In B, pooled data shows mean standardized ERK-1 phosphorylation of samples treated with HGF plus SPARC relative to HGF alone ± S.E. (n = 4). p values indicate statistical analysis using one-way ANOVA.