Multiple sequence alignments of PSA2 orthologs and homologs.
A, alignment of PSA2 orthologs. The alignment was generated with ClustalW. The DnaJ-like cysteine-rich domain predicted by InterPro (IPR001305) is marked with the four predicted zinc binding motifs underlined. The region used for antibody production is outlined in red. The pair of arginines characteristic of proteins that engage the TAT thylakoid import pathway are marked as is the predicted cleavage site that separates the targeting peptide from the mature protein. At-PSA2 corresponds to Arabidopsis thaliana gene model AT2G34860.1. Cr-PSA2 corresponds to Chlamydomonas reinhardtii gene model Cre11.g475850.t1.2. Os-PSA2 corresponds to Oryza sativa gene model Os08g36140.1. Pa-PSA2 corresponds to Populus alba gene model POPTR_0001s04720.1. Zm-PSA2 corresponds to Zea mays gene model GRMZM2G021687_P01. B, alignment of PSA2, LQY1, and BSD2 orthologs in maize and Arabidopsis. ZmBSD2 is GRMZM2G062788, AtBSD2 is AT3G47650, AtLQY1 is AT1G75690, and ZmLQY1 is GRMZM2G071996. Amino acids are colored according to the RasMol convention.