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UPLC DAD/APCI-MS analysis of vitamin K1 and its epoxide detected in the Caco-2 cellular compartment extract after a 24-h incubation with 2.5 μm micellar vitamin K1. A, DAD chromatogram of the Caco-2 cellular compartment extract at 270 nm. mAU, milliabsorbance units. Compound 1, vitamin K1 epoxide (retention time = 10.8 min); compound 2, vitamin K1 (retention time = 11.8 min). B, MS (a) and MS2 spectra (b) of vitamin K1 (compound 2). C, MS spectrum of vitamin K1 epoxide (compound 1).
