FIGURE 6.

PEDF up-regulates Fas-L expression via p53. A, cells were transfected with p53-pcDNA3.1 (+) for 48 h. The protein levels of p53 and Fas-L were measured by Western blot analysis using antibodies for p53 and Fas-L, respectively. B, PEDF increased the expression of p53 and p-p53. The protein levels of p53 and p-p53 were detected by Western blot analysis. C, cells were transfected with p53 siRNAs (sip53–1/2/3) or a nonspecific control siRNA (siCTRL) for 48 h. siRNA-mediated depletion of p53 was examined by Western blot analysis. D, knockdown of p53 was performed 24 h prior to the overexpression of PEDF. Western blot analysis was used to determine the protein levels of p53, PEDF, and Fas-L. Data are representative of three independent experiments.