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Elution profile of analytical size-exclusion chromatography. A Superdex 200 HP 16/600 column was used and chromatography was carried out as described elsewhere (22). Seven milligrams of Ni-NTA-purified MS dioxygenase was loaded onto the column. The retention time of 228 min corresponds to 43 kDa (expected size for MS dioxygenase dimer including His6 tag: 46 kDa).
