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. 2014 Sep 16;289(44):30800–30809. doi: 10.1074/jbc.M114.579730

TABLE 1.

Bacterial strains, plasmids and oligonucleotides (primers) used in this study

Strain, plasmid, or primer Relevant characteristic Source
Strains
    V. paradoxus B4 Wild type, MS-degrading DSM 21786
    E. coli Top10 F− mcrA Δ(mrr-hsdRMS-mcrBC) rpsL nupG Φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK endA1 Invitrogen
    E. coli BL21(DE3) pLysS F− ompT hsdSB (rB− mB−) gal dcm (DE3)/pLysS (Cmr) Novagen
Plasmids
    pET-23a(+) pBR322 ori, f1 ori His6; Apr T7lac Novagen
Primers
    Vp41240_NdeI_fw AAAACATATGAGCACCCTGTCCGATCCAC Eurofins MWG Operon, Ebersberg, Germany
    Vp41240_HindIII_rv AAAAAGCTTGCGGCGCGTCTGCACG Eurofins MWG Operon, Ebersberg, Germany