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. 2014 Sep 16;289(44):30800–30809. doi: 10.1074/jbc.M114.579730

TABLE 2.

Kinetic data obtained for mercaptosuccinate dioxygenase of V. paradoxus B4 in comparison to bacterial cysteine dioxygenases of B. subtilis, B. cereus, and S. coelicolor

Thiol dioxygenase Substrate Km Specific activity kcat kcat/Km
mm μmol min1 mg1 s1 mm1 s1
Cdo YubC (B. subtilis) 3.0a 1.0a 0.39a 0.1a
Cdo BC2617 (B. cereus) 5.7a 4.4a 2.0a 0.4a
Cdo SCO3035 (S. coelicolor) 1.2a 0.8a 0.33a 0.3a
Cdo SCO5772 (S. coelicolor) 3.8a 0.7a 0.30a 0.1a
MS dioxygenase (V. paradoxus) 0.4 20.0 7.7 19.2

a Values from Dominy and colleagues (15) obtained via HPLC measurements.