FIGURE 3.

Co-expression of TgMyoA with the chaperone TgUNC in Sf9 cells produces soluble heavy chain. Sf9 cells were co-infected with recombinant baculovirus coding for the TgMyoA heavy chain (HC) and its light chain TgMLC1. The Western blot shows the total (T) and soluble (S) protein fractions after 72-h infection in the absence (left two lanes) or presence (right two lanes) of co-expressed TgUNC. TgUNC was detected using anti-Myc antibody, whereas TgMyoA heavy chain was detected with anti-FLAG antibody.