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. 2014 Nov 20;21(15):2143–2148. doi: 10.1089/ars.2014.6072

FIG. 3.

FIG. 3.

Analysis of 3NT-αSyn in patients, controls and subjects with thyroidectomy. (A) Representative immunoblots of 3-nitrotyrosine-α-synuclein (after two monoclonal antibodies or mAb against 3NT- αSyn with nitrosylation of tyrosine residues at the carboxyl terminus or 3NT-αSyn Tyr125/136 mAb and the amine terminus or 3NT-αSyn Tyr39 mAb) and wild α-synuclein in early disease patients, controls, and subjects with thyroidectomy. (B) Scion density units of immunoblot bands of 3-nitrotyrosine-α-synuclein (after two monoclonal antibodies or mAb against nitrosylated αSyn with nitrosylation of tyrosine residues at the carboxyl terminus or 3NT-αSyn Tyr125/136 mAb and the amine terminus or 3NT-αSyn Tyr39 mAb) and total α-synuclein in early disease patients, controls, and subjects with thyroidectomy. Mean±SEM, *p<0.05 versus the other bands of the same group; #p<0.01 versus the same bands of controls and early disease patients (Newman–Keuls test). 3NT-αSyn Tyr125/136, 3-nitrotyrosine-α-synuclein with nitrosylated Tyr125/136 residues; 3NT-αSyn Tyr39, 3-nitrotyrosine-α-synuclein with nitrosylated Tyr39 residue; αSyn; α-synuclein; mAb, monoclonal antibody.