FIG. 4.

Evidence for cell proliferation on scaffolds. (A) Data on ³H-thymidine uptake assay. The adhesion of fibroblasts on bare PLGC and hybrid PLGC scaffolds within 2 h and proliferated cell numbers after 7 d, 14 d, and 28 d of culture are shown graphically. Bars represent mean±SD, (n=3). *p<0.05. (B, C) Micrograph of actin-stained cytoskeleton, counter stained with Hoechst for nucleus of fibroblast after 7 d of culture on bare PLGC scaffold and on hybrid scaffold, respectively. On bare PLGC fibers, cells were found aligned to the direction polymer fibers only. On the hybrid scaffold, cells were found in all directions, and across the fibers as well. Scale bar=100μm.