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. 2014 Nov 20;21(15):2126–2142. doi: 10.1089/ars.2013.5565

FIG. 10.

FIG. 10.

Sulforaphane increases the Nrf2 response and protects rat kidneys from aldosterone-induced DNA damage. Evaluation of immunohistochemical staining on paraffin-embedded kidney cortex and medulla sections for Nrf2 activation. The ratio of positive/negative nuclei was quantified by Cell Profiler within eight visual fields of cortex (A) and medulla (B) of the kidney. Evaluation of immunohistochemical staining on paraffin-embedded kidney cortex and medulla sections for γ-H2AX activation. The ratio of positive/negative nuclei was quantified by Cell Profiler within eight visual fields of the cortex (C) and medulla (D) of the kidney. (E) DNA damage measured by the comet assay in kidney cells extracted from kidneys of all groups. Results are shown as percentage of DNA in the tail. (A–E) Shown are mean values±SEM, *p≤0.05 versus the control group, #p≤0.05 versus the aldosterone-treated group, tested by ANOVA with subsequent post-hoc comparisons by Scheffé.