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. 2014 Nov 20;21(15):2126–2142. doi: 10.1089/ars.2013.5565

FIG. 3.

FIG. 3.

Expression of Nrf2-regulated proteins in LLC-PK1 cells. The expression of Nrf2-regulated proteins was detected by Western blot in cells incubated in the absence (0) and the presence of 10 (A, C, E) and 100 nM (B, D, F) aldosterone for the indicated time points. Protein levels of (A, B) TRX (71 kDa), (C, D) SOD (16 kDa), and (E, F) HO-1 (32 kDa) were measured and referred to the α-tubulin (50 kDa) content as a loading control. (A–F) Shown is the median±the standard error of the median, *p≤0.05 versus time point 0, evaluated by Mann–Whitney test. SOD, superoxide dismutase; TRX, thioredoxin; HO-1, heme oxygenase-1.