Aldosterone affects oxidative DNA damage levels in LLC-PK1 cells. (A) Cells were stained for DSBs with an antibody against γ-H2AX. Shown are representative pictures of control cells and cells treated with 100 nM aldosterone after the indicated time points. Pictures were taken using a fluorescence microscope: Quantifications were done by measuring gray values of ∼150 cells treated with either 10 nM
(B) or 100 nM
(C) aldosterone for the indicated time spans with ImageJ. (D) LLC-PK1 cells stained for 8-oxodG lesions. Shown are representative pictures of control cells and cells treated with 10 nM aldosterone after the indicated time points. Pictures were taken by a TCS SP5 laser scanning confocal microscope. Quantifications were done by measuring gray values of ∼100 cells treated with either 10 nM
(E) or 100 nM
(F) aldosterone for the indicated time spans with ImageJ. (B, C, E, F) Shown is the median±the standard error of the median, *p≤0.05 versus the control group, evaluated by Mann–Whitney test. DSBs, double strand breaks. 8-oxodG, 7,8-dihydro-8-oxo-guanine.